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1996-03-09
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Document 0159
DOCN M9650159
TI Human cytomegalovirus (CMV) DNA in plasma reflects quantity of CMV DNA
present in leukocytes.
DT 9605
AU Zipeto D; Morris S; Hong C; Dowling A; Wolitz R; Merigan TC; Rasmussen
L; Division of Infectious Diseases, Stanford University School of;
Medicine, California 94305, USA.
SO J Clin Microbiol. 1995 Oct;33(10):2607-11. Unique Identifier : AIDSLINE
MED/96087195
AB A quantitative DNA amplification assay for human cytomegalovirus (CMV)
DNA has been used to evaluate the relationship between quantities of CMV
DNA in plasma and those in infected leukocytes (WBC) from human
immunodeficiency virus-infected patients. The target sequence for DNA
amplification was a region of the immediate-early 1 gene of CMV. The
quantitation assay uses an internal control that is coamplified with
each patient sample DNA and contains a sequence for detection by
colorimetric hybridization with the same bases, but in different order
than in the CMV immediate-early 1 region used for hybridization of
amplified patient sample DNA. Results showed that patients with CMV
disease had more CMV DNA in both WBC and plasma than those without
disease. However, in this study, copy numbers of CMV DNA in WBC were
higher than those in plasma. The gB and gH variants were the same in
plasma and WBC.
DE Base Sequence Comparative Study Cytomegalovirus
Infections/*BLOOD/ETIOLOGY/PATHOLOGY DNA, Viral/BLOOD/*ISOLATION &
PURIF Genotype Human HIV Infections/*COMPLICATIONS
Leukocytes/*VIROLOGY Molecular Sequence Data Polymerase Chain Reaction
Semen/CYTOLOGY/VIROLOGY Support, Non-U.S. Gov't Support, U.S. Gov't,
P.H.S. JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).